Journal: OncoTargets and Therapy

Article Title: Comprehensive Analysis Identifies Hsa_circ_0058191 as a Potential Drug Resistance Target in Multiple Myeloma

doi: 10.2147/OTT.S505074

Figure Lengend Snippet: Screening and validation of abnormally expressed circRNAs in multiple myeloma. ( a ) A volcanic map showing 360 up-regulated and 438 down-regulated differentially expressed circRNAs in the circRNA microarray determined based on (|log2fold change|> 1.5, p < 0.05) as selection thresholds. ( b ) The clustering heatmap indicating that the bortezomib-resistant samples were clustered together, effectively separating them from healthy donor samples. ( c ) The genome map displaying the distribution of circRNAs across different chromosomes, where each horizontal line represents a chromosome, and the red dots above the line indicate the number of circRNAs on that chromosome. ( d ) The Venn diagram illustrates the intersecting genes of differentially expressed circRNAs in Multiple Myeloma from the microarray, GSE208782, and GSE133058 datasets. ( e ) The model diagram of hsa_circ_0058191. Sanger sequencing illustrating the junction point of hsa_circ_0058191. ( f ) The qRT-PCR results showing that hsa_circ_0058191 was significantly upregulated in the bortezomib-resistant group compared to the bortezomib-sensitive MM patients.

Article Snippet: Using the Arraystar Human circRNA Array (V2.0) and Agilent Feature Extraction software (version 11.0.1.1), we performed a comprehensive array analysis on whole blood samples from five bortezomib-resistant patients and healthy donors.

Techniques: Biomarker Discovery, Microarray, Selection, Sequencing, Quantitative RT-PCR

Journal: Molecular Therapy. Nucleic Acids

Article Title: The circular RNA Ataxia Telangiectasia Mutated regulates oxidative stress in smooth muscle cells in expanding abdominal aortic aneurysms

doi: 10.1016/j.omtn.2023.08.017

Figure Lengend Snippet: Circular RNAs are deregulated in human abdominal aortic aneurysm (A) Volcano plot depicting downregulated (51, blue) and upregulated (40, red). Circular RNAs (circRNAs) in human elective human abdominal aortic aneurysm (eAAA, n = 11) vs . control (CTRL, n = 6) aorta specimens, as resulted by array experiments. Log2 fold change and -log10 p value are plotted on the x and y axes, respectively. IDs of circRNAs meant for a first round of validation are highlighted. Statistics: unpaired t test; p values <0.05 are considered significant. (B) Pie chart illustrating the proportion of exonic (89.8%), intronic (5.7%), sense overlapping (3.4%), and antisense (1.1%) array-identified differentially expressed circRNAs. Absolute numbers are further indicated for each group. (C) Real-time quantitative PCR (qPCR) validation of hsa_circ_0005660 (c NFIX ), hsa_circ_0003641 (c ATM ), hsa_circ0042103 (c MYOCD ), hsa_circ003218 (c BMPR2 ), hsa_circ0004771 (c NRIP1 ), and hsa_circ0005615 (c NFATC3 ) differential expression in human eAAA (N = 8) and CTRL (N = 4) aortas. 2 –ddCT was calculated by normalizing on RPLPO . Data are represented as mean ± SEM. Statistics: unpaired t test; p values <0.05 are considered significant. NS, not significant; eAAA, elective AAA.

Article Snippet: The resulting labeled cDNA was then purified and 1 μg was fragmented, heated, and subsequently hybridized with an 8 × 15k commercially available array chip displaying 13,617 human circRNAs (Arraystar, no. AS-S-CR-H-V2.0) for 17 h at 65°C in an Agilent Hybridization Oven.

Techniques: Control, Biomarker Discovery, Real-time Polymerase Chain Reaction, Quantitative Proteomics

Journal: Oncology Reports

Article Title: Dysregulated circular RNAs are closely linked to multiple myeloma prognosis, with circ_0026652 predicting bortezomib-based treatment response and survival via the microRNA-608-mediated Wnt/β-catenin pathway

doi: 10.3892/or.2022.8410

Figure Lengend Snippet: PCA plots, heatmap analysis and volcano plots. (A) PCA plots and (B) heatmap analysis of circRNA profiles between eight CR patients and eight NR patients. (C) Volcano plots of dysregulated circRNAs between eight CR patients and eight NR patients. PCA, principal component analysis; circRNA, circular RNA; CR, complete response; NR, no response.

Article Snippet: Subsequently, the samples were hybridized using Arraystar Human CircRNA Array v1 (AS-S-CR-H-V2.0; Arraystar.) and Gene Expression Microarray Hybridization Kit (Agilent Technologies, Inc.), according to the manufacturer's instructions in an Agilent Hybridization Oven (Agilent Technologies, Inc.).

Techniques:

Journal: Oncology Reports

Article Title: Dysregulated circular RNAs are closely linked to multiple myeloma prognosis, with circ_0026652 predicting bortezomib-based treatment response and survival via the microRNA-608-mediated Wnt/β-catenin pathway

doi: 10.3892/or.2022.8410

Figure Lengend Snippet: Enrichment analyses for dysregulated circRNAs. (A) GO and (B) KEGG enrichment analyses based on located genes of dysregulated circRNAs in patients with MM. (C) GO and (D) KEGG/HP/DOID enrichment analyses based on target miRNAs of dysregulated circRNAs in patients with MM. CircRNA, circular RNA; GO, Gene Ontology; KEGG, Kyoko Encyclopedia of Genes and Genomes; HP, Human Phenotype Ontology; DOID, Disease Ontology Identification; miRNAs, microRNAs; MM, multiple myeloma.

Article Snippet: Subsequently, the samples were hybridized using Arraystar Human CircRNA Array v1 (AS-S-CR-H-V2.0; Arraystar.) and Gene Expression Microarray Hybridization Kit (Agilent Technologies, Inc.), according to the manufacturer's instructions in an Agilent Hybridization Oven (Agilent Technologies, Inc.).

Techniques:

Journal: Oncology Reports

Article Title: Dysregulated circular RNAs are closely linked to multiple myeloma prognosis, with circ_0026652 predicting bortezomib-based treatment response and survival via the microRNA-608-mediated Wnt/β-catenin pathway

doi: 10.3892/or.2022.8410

Figure Lengend Snippet: CircRNA-miRNA interaction network. Ten candidate circRNAs with their potential target miRNAs. Red represents downregulated circRNAs, while green indicates upregulated circRNAs. CircRNA/circ, circular RNA; miRNA/miR, microRNA.

Article Snippet: Subsequently, the samples were hybridized using Arraystar Human CircRNA Array v1 (AS-S-CR-H-V2.0; Arraystar.) and Gene Expression Microarray Hybridization Kit (Agilent Technologies, Inc.), according to the manufacturer's instructions in an Agilent Hybridization Oven (Agilent Technologies, Inc.).

Techniques:

Journal: Oncology Reports

Article Title: Dysregulated circular RNAs are closely linked to multiple myeloma prognosis, with circ_0026652 predicting bortezomib-based treatment response and survival via the microRNA-608-mediated Wnt/β-catenin pathway

doi: 10.3892/or.2022.8410

Figure Lengend Snippet: Ten candidate circRNAs between CR patients and NR patients. Comparisons of (A) circ_0026652, (B) circ_0068708, (C) circ_0088128, (D) circ_0001566, (E) circ_0005327, (F) circ_0031113, (G) circ_0083587, (H) circ_0005552, (I) circ_0007171 and (J) circ_0007521 expression levels between CR patients (n=16) and NR patients (n=17). CircRNA/circ, circular RNA; CR, complete response; NR, no response.

Article Snippet: Subsequently, the samples were hybridized using Arraystar Human CircRNA Array v1 (AS-S-CR-H-V2.0; Arraystar.) and Gene Expression Microarray Hybridization Kit (Agilent Technologies, Inc.), according to the manufacturer's instructions in an Agilent Hybridization Oven (Agilent Technologies, Inc.).

Techniques: Expressing

Journal: Oncology Reports

Article Title: Dysregulated circular RNAs are closely linked to multiple myeloma prognosis, with circ_0026652 predicting bortezomib-based treatment response and survival via the microRNA-608-mediated Wnt/β-catenin pathway

doi: 10.3892/or.2022.8410

Figure Lengend Snippet: Ten candidate circRNAs between CR patients and non-CR patients. Comparisons of (A) circ_0026652, (B) circ_0068708, (C) circ_0088128, (D) circ_0001566, (E) circ_0005327, (F) circ_0031113, (G) circ_0083587, (H) circ_0005552, (I) circ_0007171 and (J) circ_0007521 expression levels between CR patients (n=16) and non-CR (n=44) patients. CircRNA/circ, circular RNA; CR, complete response.

Article Snippet: Subsequently, the samples were hybridized using Arraystar Human CircRNA Array v1 (AS-S-CR-H-V2.0; Arraystar.) and Gene Expression Microarray Hybridization Kit (Agilent Technologies, Inc.), according to the manufacturer's instructions in an Agilent Hybridization Oven (Agilent Technologies, Inc.).

Techniques: Expressing

Journal: Oncology Reports

Article Title: Dysregulated circular RNAs are closely linked to multiple myeloma prognosis, with circ_0026652 predicting bortezomib-based treatment response and survival via the microRNA-608-mediated Wnt/β-catenin pathway

doi: 10.3892/or.2022.8410

Figure Lengend Snippet: Ten candidate circRNAs between ORR patients and non-ORR patients. Comparisons of (A) circ_0026652, (B) circ_0068708, (C) circ_0088128, (D) circ_0001566, (E) circ_0005327, (F) circ_0031113, (G) circ_0083587, (H) circ_0005552, (I) circ_0007171 and (J) circ_0007521 expression levels between ORR patients (n=43) and non-ORR (n=17) patients. CircRNA/circ, circular RNA; ORR, objective response rate.

Article Snippet: Subsequently, the samples were hybridized using Arraystar Human CircRNA Array v1 (AS-S-CR-H-V2.0; Arraystar.) and Gene Expression Microarray Hybridization Kit (Agilent Technologies, Inc.), according to the manufacturer's instructions in an Agilent Hybridization Oven (Agilent Technologies, Inc.).

Techniques: Expressing

Journal: Oncology Reports

Article Title: Dysregulated circular RNAs are closely linked to multiple myeloma prognosis, with circ_0026652 predicting bortezomib-based treatment response and survival via the microRNA-608-mediated Wnt/β-catenin pathway

doi: 10.3892/or.2022.8410

Figure Lengend Snippet: Associations between candidate circRNAs and PFS and OS. Associations between circ_0026652, circ_0068708, circ_0088128, circ_0001566, circ_0005327, circ_0031113, circ_0083587, circ_0005552, circ_0007171 and circ_0007521 expression levels and (A-J) PFS and (K-T) OS. CircRNA/circ, circular RNA; PFS, progressive-free survival; OS, overall survival.

Article Snippet: Subsequently, the samples were hybridized using Arraystar Human CircRNA Array v1 (AS-S-CR-H-V2.0; Arraystar.) and Gene Expression Microarray Hybridization Kit (Agilent Technologies, Inc.), according to the manufacturer's instructions in an Agilent Hybridization Oven (Agilent Technologies, Inc.).

Techniques: Expressing